Twelve-week treadmill endurance training in mice is associated with upregulation of interleukin-15 and natural killer cell activation and increases apoptosis rate in Hepa1-6 cell-derived mouse hepatomas

Regular exercise reduces the risk of malignancy and decreases the recurrence of cancer. However, the mechanisms behind this protection remain to be elucidated. Natural killer (NK) cells are lymphocytes of the innate immune system, which play essential roles in immune defense and effectively prevent cancer metastasis. Physical exercise can increase the activity of NK cells. Interleukin-15 (IL-15) is the best-studied cytokine activator of NK cells, and it was shown to have many positive functional effects on NK cells to improve antitumor responses. The aim of this study was to clarify the possible important mechanisms behind endurance exercise-induced changes in NK cell function, which may be highly correlated with IL-15. An animal model was used to study IL-15 expression level, tumor volume, cancer cell apoptosis, and NK cell infiltration after treadmill exercise. Although IL-15 was highly expressed in skeletal muscle, treadmill exercise further elevated IL-15 levels in plasma and muscle (P<0.05). In addition, tumor weight and volume of tumor-bearing mice were decreased (P<0.05), and liver tumor cell apoptosis was increased after 12 weeks of treadmill exercise (P<0.05). NK cell infiltration was upregulated in tumors from treadmill exercise mice, and the level of interferon-gamma (IFN-γ) and IL-15 were higher than in sedentary mice (P<0.05). The study indicated that regular endurance training can reduce cancer risk, which was related to increased IL-15 expression, activation of the immune killing effect of NK cells, and promotion of tumor cell apoptosis, which can ultimately control tumor growth.

Screening of IGF-1, NOS, FGF23 and Sclerost-Targeted Mechanoresponsive MicroRNAs / 医用生物力学

Objective To screen the secretory factor-related, mechanoresponsive microRNAs (miRNA) of osteocytes. Methods Cyclic mechanical tensile strain (ε=2.5,f=0.5 Hz) was applied to osteocytes and osteoblasts cultured in vitro respectively, and the differentially expressed miRNAs only in the osteocytes were screened out by using miRNA chip. Through bioinformatics technology, in these differentially expressed miRNAs, the target genes of secretory factors including insulin-like growth factor-1(IGF-1), nitric oxide synthesase (NOS), fibroblast growth factor 23 (FGF23) and sclerostin (SOST) were further screened out. Then the selected miRNAs were compared with the biochip detected, differentially expressed miRNAs in femur bone of the mice which were trained on treadmill, and four of these miRNAs were randomly selected for quantitative PCR verification. Results For the 77 differentially expressed miRNAs only in the mechanically strained osteocytes in vitro, 22 miRNAs whose target genes were the 4 secreted factors (IGF-1, NOS, FGF23 and SOST), were screened out. Moreover, a total of 11 miRNAs in the 22 miRNAs were differentially expressed in femur bone of the treadmill trained mice with the same trend as those in osteocytes in vitro, and the randomly selected miR-361-3p, miR-3082-5p, miR-6348 and miR-706 were confirmed to be differentially expressed with the same trend in femur bone and osteocytes. Conclusions These mechanoresponsive miRNAs differentially expressed only in osteocytes, such as miR-361-3p, miR-3082-5p, miR-6348 and miR-706, probably influence osteoblastic differentiation or bone metabolism through regulating the secretory factors.

Effect of Early Treadmill Exercise and Massage on Activative Factors of Muscle Satellite Cells after Acute Injury of Skeletal Muscle in Rats / 中国康复理论与实践

Objective:To study the effect of treadmill exercise and massage shortly after acute injury on expression of key growth factor in muscle satellite cells activation, insulin-like growth factor-1 (IGF-1), and mitogen-activated protein kinase (MAPK) / mitogen-activated protein kinase kinase (MEK) / extracellular regulated protein kinases (ERK)1/2 signaling pathways in muscle satellite cell in rats. Methods:A total of 40 SPF male adult Sprague-Dawley rats were randomly divided into groups A (n = 8), B (n = 8), C (n = 8), D (n = 8) and E (n = 8). Group A did not receive any treatment, while the other rats were contused the gastrocnemius muscle with self-made impactor. Group B received no intervention, groups C and D received massage and treadmill exercise shortly after injury, respectively, while group E received both treadmill exercise and massage shortly after injury. As the model was established, samples of gastrocnemius were obtained from all the rats 24 hours after injury, and observed under HE staining, detected the expression of MyoD1 and MyoG mRNA with reverse transcription polymerase chain reaction (RT-PCR), and expression of IGF-1, p-MAPK, p-MEK and p-ERKI/2 protein with Western blotting. Results:The expression of MyoD1 mRNA was more in groups C, D and E than in group B, which was the most in group C; less expression of MyoG mRNA, which was the most in group E (P < 0.05). The expression of p-MAPK, p-MEK and p-EPK1/2 was more in groups C, D and E than in group B, which was the most in group D (P < 0.05). The expression of IGF-1 increased in group C compared with that in group B (P < 0.05), and it decreased in group D (P < 0.05). Conclusion:Early intervention of treadmill exercise and massage may promote the activation of muscle satellite cells in different ways.

Treadmill Exercise Improves Motor Function by Suppressing Purkinje Cell Loss in Parkinson Disease Rats / 대한배뇨장애요실금학회지

PURPOSE: Rotenone is the most widely used neurotoxin for the making Parkinson disease (PD) animal model. The neurodegenerative disorder PD shows symptoms, such as slowness of movements, tremor at resting, rigidity, disturbance of gait, and instability of posture. We investigated whether treadmill running improves motor ability using rotenone-caused PD rats. The effect of treadmill running on PD was also assessed in relation with apoptosis of cerebellar Purkinje cells. METHODS: Treadmill running was applied to the rats in the exercise groups for 30 minutes once a day for 4 weeks, starting 4 weeks after birth. We used rota-rod test for the determination of motor coordination and balance. In this experiment, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, immunohistochemistry for calbindin, glial fibrillary acidic protein (GFAP), Iba-1, and western blot analysis for Bax and Bcl-2 were performed. RESULTS: Treadmill running enhanced motor balance and coordination by preventing the loss of Purkinje cells in the cerebellar vermis. Treadmill running suppressed PD-induced expression of GFAP-positive reactive astrocytes and Iba-1-positive microglia, showing that treadmill running suppressed reactive astrogliosis and microglia activation. Treadmill running suppressed TUNEL-positive cell number and Bax expression and enhanced Bcl-2 expression, demonstrating that treadmill running inhibited the progress of apoptosis in the cerebellum of rotenone-induced PD rats. CONCLUSIONS: Treadmill running improved motor ability of the rotenone-induced PD rats by inhibiting apoptosis in the cerebellum. Apoptosis suppressing effect of treadmill running on rotenone-induced PD was achieved via suppression of reactive astrocyte and inhibition of microglial activation.

The clinical study on the effect of Tongyang-Kuanxiong guanule (TKG) for the stable angina pectoris patients with the type of Qi and phlegm stasis / 国际中医中药杂志

Objective To observe the effect of Tongyang-Kuanxiong guanule combined with conventional western medicine for the stable angina pectoris (SAP) with qi and phlegm and blood stasis syndrome. Methods Eighty-six SAP patients who met the inclusion criteria were randomly divided into two groups according to the digital table method, with 43 cases in each group. The control group was treated with routine Western medical therapy. The observation group was given Tongyang-Kuanxiong guanule on the basis of the control group. Both groups were treated for 28 days. The TCM syndromes and angina pectoris scores were evaluated before and after treatment. The treadmill exercise test was used to observe the duration of exercise, the metabolic equivalent, the maximum value of ST segment depression, the heart rate at maximum load, the duration of ST segment depression. And the adverse reactions were recorded. Results The total effective rate was 88.4％ (38/43) in the observation group and 67.4％ (29/43) in the control group. The difference between the two groups was statistically significant (χ2=5.549, P=0.018). After treatment, the duration of exercise (9.5 ± 1.2 min vs. 8.6 ± 1.2 min, t=3.371), metabolic equivalent (10.8 ± 2.5 METs vs. 9.2 ± 2.4 METs, t=3.084) in the observation group were significantly higher than those in the control group (P<0.01 or P<0.05). The largest degree of ST segment changes (1.2 ± 0.6 mV vs. 1.9 ± 0.6 mV, t=5.484), and the duration of ST segment descent (125.0 ± 94.5 s vs. 162.4 ± 96.9 s, t=1.795) in the observation group were significantly lower than those in the control group (P<0.01 or P<0.05). Thefrequency of angina attacks (9.7 ± 1.6 times per week vs. 17.3 ± 2.1 times per week, t=18.778), duration of attacks (3.2 ± 1.4 min vs. 6.4 ± 1.5 min, t=10.146) consumption of nitroglycerin (1.3 ± 0.8 mg vs. 2.5 ± 0.8 mg, t=7.624) and Symptom score (8.3 ± 2.8 vs. 12.5 ± 3.1, t=6.593) in the observation group were significantly lower than the control group (P<0.01). Conclusions The Tongyang-Kuanxiong guanule can significantly improve the exercise tolerance of SAP patients with qi and phlegm and blood stasis, reduce myocardial oxygen consumption, improve angina pectoris and reduce the number of angina attacks.

Effect of Treadmill Exercise on Expression of Matrix Metalloproteinase-2 in Rats with Traumatic Brain Injury / 中国康复理论与实践

Objective To determine the effect of treadmill exercise on expression of matrix metalloproteinase-2 (MMP-2) in rats follow-ing traumatic brain injury (TBI). Methods Fifty-five male adult Sprague-Dawley rats were randomly assigned to sham (n=15), control (n=20) and exercise (n=20) groups, the later two groups subjected to unilateral cortical contusion injury (CCI). All the rats were assessed with foot-fault test 6, 12, 18 and 24 days after CCI. Evans blue perfusion was used to evaluate the integrity of the blood-brain-barrier (BBB) 48 hours after CCI. Protein expression of MMP-2 was determined with Western blotting one week after CCI. Results The score of foot-fault test improved more in the exercise group than in the control group 12, 18 and 24 days after CCI (F>4.793, P<0.05). Evans blue extravasa-tion was less in the exercise group than in the control group (t=-8.091, P<0.001), as well as the expression of MMP-2 (t=-13.12, P<0.001). Conclusion Early treadmill exercise can improve the motor function in rats with TBI, which may associate with inhibition of MMP-2 ex-pression to protect BBB integrity.

Effects of Different Interventions on Biomechanic Indexes of Femur Bones of Ovariectomized Rats with Osteoporosis / 中国运动医学杂志

Objective To investigate the effects of different interventions on the biomechanic indexes in femur bones of ovariectomized rats with osteoporosis.Methods Eighty healthy female Sprague-Dawley rats,aged 3 months,were randomly divided into an ovariectornized(OVX,n=68)group and a sham-operation(Sham,n=12)group.At the 11th week after the operation,the OVX group was further randomly divided into a control group(n=11),an estradiol group(E2,n=10),a genisteine group(G,n=10),a treadmill exercise group(TE,n=10),a lithium chloride group(LiCl,n=10)and a whole-body vertical vibration group (WBVV,n=10).Then the rats began to receive different interventions as their group names implied.At the end of 8 weeks,their right femurs were isolated and the biomechanic parameters were detected using the three point bending test.Results (1)Both the maximum load and elastic load in E2,G,TE,WBVV and LiC1 groups were significantly higher than that of the control group,while no significant differences were seen in maximum deflection and elastic deflection.(2)The elastic stress,maximum strain,and elasticity modulus in E2,G,and WBVV groups were significantly higher than that of the control group,while no significant differences were seen in maximum stress and elastic strain among them.The maximum stress,elastic stress,maximum strain,and elasticity modulus in TE group were significantly higher than the control group while no significant differences were seen in the elastic strain.The maximum stress,elastic stress,and elasticity modulus in LiC1 group were significantly higher than the control group,but no significant differences were seen in maximum strain and elastic strain between them.Conclusion E2,genistein,treadmill exercise,whole-body vertical vibration,and LiC1 have quite similar effects on structural mechanics indexes of femurs in OVX rats with osteoporosis,but they have different impacts on the material mechanics indexes.

Comparison between treadmill exercise test and dynamic electrocardiography in diagnosing coronary heart disease:A meta?analysis / 实用医学杂志

Objective To systematically evaluate the diagnostic value of treadmill exercise test (TET) compared with dynamic electrocardiography (DCG). Methods Databases including Cochrane library, MEDLINE,EMbase,Google Scholar and CNKI were retrieved to collect randomized clinical trials (RCT) and controlled clinical trials(CCT)focusing on comparison of TET and DCG. After screening according to the inclusion and exclusion criteria,all articles received Jadad quality assessment,and were analyzed using Review Manager Version 5.3 software(The Cochrane Collaboration,Oxford,United Kingdom). Results 9 papers with a total of 667 patients who underwent coronary angiography(CAG),TET and DCG were eventually enrolled. Results of the present Meta?analysis were as below.(1)Sensitivity:9 trials(n = 552)suggested that the sensitivity is 80.62% in TET Group versus 72.64%in DCG Group[RR=1.11,95%CI(1.04~1.18),P=0.002]. The difference was statistically significant.(2)Specificity:7 trails(n=126)indicated that no statistical significance[RR=0.86,95%CI(0.71~1.04),P=0.11]was found in specificity when comparing TET(68/115)and DCG(87/126). Conclusion TET has higher sensitivity but similar specificity in comparison with DCG,and it turns out to be a simple and feasible inspecting method in diagnosing coronary heart disease.

Analysis of the influencing factors on the false negative of electrocardiogram treadmill exercise test in pa-tients with coronary heart disease / 中国综合临床

Objective To study the influencing factors on the false negative of electrocardiogram tread?mill exercise test.Methods One hundred and seventy cases coronary heart disease( CHD) patients with coro?nary angiography and carried out electrocardiogram treadmill exercise test from January 2012 to June 2015 in Cardiology Department of the People’ s Hospital of Yanchang County were selected as research objects,including 80 cases of false negative and 90 of positive.The clinical data of two groups were analyzed retrospectively.Results (1)Single factor analysis:CAG results in the false negative group showed that there were 54 cases(67.50%) of single vessel stenosis,14 cases(17.50%) of two vessel stenosis,12 cases(15.00%) of three vessel stenosis,in positive group there were 48 cases(53.33%),34 cases(37.78%) and 8 cases(8.89%) respectively,the differ?ences between the two groups was significant(χ2=8.93,P<0.05).The ST segment of the coronary artery stenosis in the false negative group was 19 cases(23.75%),83 cases (92.22%) in the positive group.There were 61 ca?ses (76.25%) and 7 cases(7.78%) with good collateral circulation in the coronary artery of the two groups,re?spectively,the difference between the two groups was significant(χ2=82.74,P<0.01).There were significant differences in terms of left anterior descending artery(LCX),left anterior descending branch(LAD),left main trunk(LM) diameter between the two groups((3.58±0.95) mm vs. (3.47±0.15) mm,(4.25±0.11) mm vs. (3.94±0.17) mm,(4.58±0.37) mm vs. (4.37±0.26) mm;t=2.91,6.80,2.06;P<0.05).(2)Considering false negative and positive as a dependent variable,and items with significance level including the number of narrow blood vessels,the diameter of coronary artery,LCX,LAD and LM diameters as independent variables in the sin?gle factor analysis,logistic regression analysis was carried out to find that LAD had significant difference in diam?eter,which was independent risk factor for the development of electrocardiogram exercise test false negatives( OR=16.95,95%CI 3.58-80.27,P<0.01).Conclusion False negative is associated with coronary artery diameter for coronary heart disease patients after electrocardiogram treadmill exercise test.The wider coronary diameter is, the easier to produce false negative results in exercise treadmill test.

Effects of early-stage exercise on functional recovery and ED1 expression after spinal cord injury / 中华物理医学与康复杂志

Objective To observe the effects of early-stage exercise on function recovery and ED1 expres-sion after spinal cord injury. Methods Twenty-two SD rats were randomly divided into a control group ( n=8) , an exercise group ( n=8) and a sham-operated group ( n=6) . The control and exercise groups received T9 spinal cord contusions. The sham-operated group received only T9 laminectomy without spinal cord injury. Two rats died within 6 hours after the operation and 3 others were abandoned because of motor function recovery just after the spi-nal cord contusion. The remaining 17 were included in the statistical analysis: a control group of 6, an exercise group of 5 and a sham-operated group of 6. BBB scoring was conducted 2 days later and then weekly until 8 weeks after the operation. The exercise group began treadmill and wheel running exercise on day 2 and continued 5 times per week until the 8th week. After paraformaldehyde perfusion, fixation and embedding, frozen spinal cord slices were Nissl stained for ED1 and GFAP immunofluorescence measurements. Results Compared with the control group, the average BBB score of the exercise group was significantly higher at each week except weeks 2 and 4. By weeks 7 and 8, the exercised rats showed 13.60 and 14.60 BBB punctuation, which means that those animals took frequent, consistent, weight-supporting plantar steps, consistent with FL-HL coordination. That motor behavior was not achieved by the control group even occasionally. In terms of the tissue morphology and immunohistochemical staining results, the empty area was not significantly reduced in the exercise group compared with the controls, but the damaged area was significantly reduced. In addition, ED1 positive cells in the lesioned area were significantly reduced in the exercised group compared with the controls. Conclusions Exercise can promote the recovery of motor function after spinal cord injury. The mechanism may involve reducing the lesioned area and inflammatory response.

Evaluation of coronary myocardial bridge by electrocardiogram and treadmill exercise test / 中国基层医药

Objective To analyze the electrocardiogram and treadmill exercise performance in patients with coronary artery myocardial bridge.Methods 176 patients with myocardial bridging diagnosed by coronary angiography were selected in the study.The performance of myocardial bridge electrocardiogram and treadmill exercise of different depth and stenosis was observed.Results Deep in myocardial bridge ischemic ST segment and T wave changes were significantly higher than superficial type (x2 =11.02,P ＜ 0.01).Systolic myocardial bridge Ⅲ grade stenosis of ischemic ST segment and T wave changes were significantly higher than grade Ⅰ and grade Ⅱ (x2 =12.78,P ＜0.01;x2 =24.45,P ＜ 0.01).The positive rate of myocardial bridge deep in the treadmill exercise test was significantly higher than the superficial type.Myocardial bridge Ⅲ grade systolic stenosis,treadmill exercise test positive rate was significantly higher than grade Ⅰ and grade Ⅱ (x2 =9.23,P ＜ 0.05;x2 =6.76,P ＜ 0.01).Conclusion Patients with myocardial bridge have various changes in the electrocardiogram and treadmill exercise,changes in deep in the type and degree of stenosis Ⅲ grade systolic are obvious.

Low-intensity treadmill exercise promotes rat dorsal wound healing / 华中科技大学学报（医学）（英德文版）

In order to investigate the promoting effect of low-intensity treadmill exercise on rat dorsal wound healing and the mechanism, 20 Sprague-Dawley rats were randomly divided into two groups: exercise group (Ex) and non-exercise group (non-ex). The rats in Ex group were given treadmill exercise for one month, and those in non-ex group raised on the same conditions without treadmill exercise. Both groups received dorsal wound operation with free access to food and water. By two-week continuous observation and recording of the wound area, the healing rate was analyzed. The blood sample was collected at day 14 post-operation via cardiac puncture for determination of the number of endothelial progenitor cells (EPCs) by flow cytometry, and the concentrations of relevant cytokines such as basic fibroblast growth factor (bFGF), endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) were measured by ELISA. The skin tissue around the wound was dissected to observe the vascular density under the microscope after HE staining, to detect the mRNA level of VEGFR2 and angiopoietin-1 (Ang-1) receptor using RT-qPCR, and protein expression of a-smooth muscle actin (αSMA) and type III collagen (ColIII) using Western blotting. It was found that the wound area in Ex group was smaller at the same time point than in non-ex group. The number of circulating EPCs was greater and the concentrations of vasoactive factors such as VEGF, eNOS and bFGF were higher in Ex group than in non-ex group. HE staining displayed a higher vessel density in Ex group than in non-ex group. Moreover, the mRNA expression of VEGFR2 and Ang-1 detected in the wound tissue in Ex group was higher than in non-ex group. Meanwhile, the protein expression of αSMA and ColIII was more abundant in Ex group than in non-ex group. Conclusively, the above results demonstrate Ex rats had a higher wound healing rate, suggesting low-intensity treadmill exercise accelerates wound healing. The present work may provide some hint for future study of treating refractory wound.

Effects of Regular Treadmill Exercise on a DNA Oxidative-Damage Marker and Total Antioxidant Capacity in Rat Hippocampal Tissue

BACKGROUND AND PURPOSE: Regular exercise can result in changes in the levels of oxidative stress in the hippocampus; however, little attention has been paid to physical-activity-induced neuronal protection to exposure to lead compounds. This study investigated the effects of regular treadmill exercise on a DNA oxidative-damage marker [8-hydroxy-2'-deoxyguanosine (8-OHdG)] and the total antioxidant capacity (TAC) of hippocampal tissue in lead-acetate exposed rats. METHODS: This study investigated the effects of 8 weeks of regular treadmill exercise on 8-OHdG and the TAC of hippocampal tissue in lead-acetate-exposed rats. Wistar rats were randomly divided into four groups: baseline, sham (control), lead, and exercise+lead. The exercise program involved running on a treadmill with increasing intensity five times a week for 8 weeks. Animals in the lead and exercise+lead groups received lead acetate at 20 mg/kg body weight intraperitoneally three times weekly for 8 weeks. Animals in the sham group received solvent (ethyl oleate) at 30 mg/kg body weight three times weekly for 8 weeks. TAC and 8-OHdG were measured by spectrophotometric and ELISA techniques, respectively. Data were analyzed by ANOVA and Tukey's post-hoc test with a significance cutoff of p≤0.05. RESULTS: The level of 8-OHdG and the TAC were significantly higher and lower, respectively, in the lead group than in the baseline and sham groups (p<0.01). However, the 8-OHdG level and TAC value in hippocampal tissue were significantly decreased and increased, respectively, in the exercise+lead group relative to the lead group (p<0.05). CONCLUSIONS: The TAC of hippocampal tissue may be directly associated with neural protection mechanisms of exercise following lead acetate injection, and the beneficial effects of regular exercise in preventing hippocampal neuronal damage could be due to decreased hippocampal oxidative stress such as reflected by a lower 8-OHdG level and increased TAC.

Exercise-induced ventricular arrhythmias and vagal dysfunction in Chagas disease patients with no apparent cardiac involvement

INTRODUCTION : Exercise-induced ventricular arrhythmia (EIVA) and autonomic imbalance are considered as early markers of heart disease in Chagas disease (ChD) patients. The objective of the present study was to verify the differences in the occurrence of EIVA and autonomic maneuver indexes between healthy individuals and ChD patients with no apparent cardiac involvement. METHODS : A total of 75 ChD patients with no apparent cardiac involvement, aged 44.7 (8.5) years, and 38 healthy individuals, aged 44.0 (9.2) years, were evaluated using echocardiography, symptom-limited treadmill exercise testing and autonomic function tests. RESULTS : The occurrence of EIVA was higher in the chagasic group (48%) than in the control group (23.7%) during both the effort and the recovery phases. Frequent ventricular contractions occurred only in the patient group. Additionally, the respiratory sinus arrhythmia index was significantly lower in the chagasic individuals compared with the control group. CONCLUSIONS : ChD patients with no apparent cardiac involvement had a higher frequency of EIVA as well as more vagal dysfunction by respiratory sinus arrhythmia. These results suggest that even when asymptomatic, ChD patients possess important arrhythmogenic substrates and subclinical disease. .

Effects of Two Kinds of Treadmill Exercise on Inflammatory Mediators in 18-30 Year Old Males / 中国康复理论与实践

@#Objective To compare the effects of short- term high- intensity interval training (HIT) and moderate continuous training (MCT) on inflammatory mediators for healthy young males. Methods 19 healthy young male volunteers were randomized into HIT group (n=6), MCT group (n=7) and no training control (CON, n=6) group. The groups of HIT and MCT accepted treadmill exercise once per 2 days to 6 times (2 weeks), and the CON group did not accepted treadmill exercise. The levels of high-sensitive C-reactive protein (hs-CRP), interleukin (IL) -1α, IL-6 and tumor necrosis factor α (TNF-α) were detected 3 days before exercise, immediately after the first exercise, 3 days after the course. Results There was no difference among groups in the level of hs-CRP, IL-1α, IL-6 and TNF-α in all the time (P>0.05). The concentration of IL-1α increased in the MCT group after the first exercise (P<0.01), and also the concentration of IL-6 in the CON group after the course (P<0.05). Conclusion It needs further srudy for the significance of HIT and MCT in the levels of inflammatory media.

The clinical application of the recovery time after ST segment depression for the diagnosis of coronary heart disease / 实用医学杂志

Objective To investigate the clinical significance of the ST segment changing in treadmill exercise test positive for the diagnosis of coronary heart disease. Methods 133 patients (hospitalized from January 2014 to January 2015) with the ST segment depression≥0.1 mm in treadmill exercise test were retrospectively analyzed. Patients were divided into the observation group and the control group according to the coronary angiography (the dividing line is artery stenosis is more than 50% or not ). ST-segment depression margin and recovery time of the two groups were compared. ST-segment depression margin and recovery time of patients with different vascular lesion number were also analyzed. Results In 133 cases, 79 cases of coronary angiography were positive(59.4%). Positive rate of ST segment recovery time(> 90 s) was 76.9% in. The maximal ST-segment depression margin and recovery time in the observation group were more than the control group, the difference was statistically significant (P 0.05). But in terms of ST-segment recovery time, the double-vessel and multivessel stenosis group were higher than the single-vessel stenosis group , and the difference was statistically significant (P< 0.05). Conclusion Clinically, patients with TET positive combined the ST segment depression and ST-segment recovery time are important hints for the diagnosis of coronary artery disease , and is worthy of promotion.

The value of Duke treadmill score in coronary heart disease / 中国基层医药

Objective To discuss the value of Duke treadmill score in the diagnosis of coronary heart disease.Methods 94 inpatients underwent treadmill test and coronary angiography,the relationship between the Duke treadmill score and the results of coronary angiography was analyzed.Results The prognostic accuracy and positive predictive value of Duke treadmill score to male and female were 88.9％,85.0％ ;those of traditional electrocardiogram ST standard were 89.6％,82.5 ％,the differences were significant (x2 =4.27,P ＜ 0.05).Conclusion The Duke treadmill score is much more significant than the traditional ST standard in the prognostic accuracy and positive predictive value of diagnosing coronary heart disease.

Analysis of treadmill exercise test on patients with left main coronary artery or proximal anterior descending obstruction / 中国综合临床

Objective To explore the significance of treadmill exercise test (TET) in identifying left main coronary artery(LMD) obstruction from proximal left anterior descending(LAD) obstruction.Methods Patients with positive in the TET of the Central Hospital of Dalian from Jan.2008 to Dec.2013 were enrolled in this study and they were divided into LMD group with 21 cases and LAD group with 35 cases according to the results of coronary angiography.Retrospectively study was conducted to analyze the exercise time,the start time of ST segment change,the lead of ST segment between groups.Results Lead aVR ST segment elevation(≥0.10 mV) was 85.7％ (18/21) in the LMD group and 62.9％ (22/35) in the LAD group.Lead aVR ST segment elevation in the LM group was ((0.16 ± 0.07) mV,higher than in the LAD group ((0.11 ± 0.07) mV ; t =2.614,P ＜ 0.05).Accompany ST segment depression of leads and cumulative ST segment depression were (5.7 ±1.7) pcs and (1.13 ±0.42) mV in LMD group,significantly higher than those of LAD group((4.6 ± ± 1.8) pcs and (0.87 ± 0.32) mV ; t =2.314,2.634,P ＜ 0.05).The occurrence rate of ST segment depression (≥0.10 mV) in leads V2 and V6 were 33.3％ (7/21) and 90.5％ (19/21) in LM group,significantly higher than that in LAD group (11.4％ (4/35) and 62.9 ％ (22/35) ; x2 =3.990,5.105 ;P ＜ 0..05).The start time of ST segment deviation of LM group was significantly earlier than that of LAD group ((199.8 ± 70.8) s vs.(273.7 ± 127.3) s;t =-2.787,P ＜ 0.01).The differences were not statistically significant in terms of the exercise time between groups ((315.0 ± 120.0) s vs.(376.1 ± 110.0) s;t =-1.948;P ＞ 0.05),the rate for achieving target heart rate(33.3％ (7/21) vs.48.6％ (17/35) ; x2 =1.244 ; P ＞ 0.05) and the Duke score ((-11.8 ±7.2) vs.(-8.4 ±6.8) ;t =-1:772;P＞0.05).Conclusion Compare with patients with the LAD disease,ST segment elevation of lead aVR is significantly higher induced by treadmill exercise test in patients with left main disease.Meanwhile,the lead accompanied with ST segment depression is significantly higher,and the start time of ST segment deviation is significantly earlier.

Effects of Two Kinds of Treadmill Exercise on Inflammatory Mediators in 18-30 Year Old Males / 中国康复理论与实践

Objective To compare the effects of short-term high-intensity interval training (HIT) and moderate continuous training (MCT) on inflammatory mediators for healthy young males. Methods 19 healthy young male volunteers were randomized into HIT group (n=6), MCT group (n=7) and no training control (CON, n=6) group. The groups of HIT and MCT accepted treadmill exercise once per 2 days to 6 times (2 weeks), and the CON group did not accepted treadmill exercise. The levels of high-sensitive C-reactive protein (hs-CRP), interleukin (IL)-1α, IL-6 and tumor necrosis factorα(TNF-α) were detected 3 days before exercise, immediately after the first exercise, 3 days after the course. Results There was no difference among groups in the level of hs-CRP, IL-1α, IL-6 and TNF-αin all the time (P>0.05). The concentration of IL-1αincreased in the MCT group after the first exercise (P<0.01), and also the concentration of IL-6 in the CON group after the course (P<0.05). Conclusion It needs further srudy for the significance of HIT and MCT in the levels of inflammatory media.